After coming off the bench to help the Blaugrana rescue a 2-2 draw against Sevilla over the weekend, the Spanish side are hopeful the club-record goal scorer will be able to feature prominently against the Serie A side.
Messi's late heroics last time out maintained their incredible unbeaten record in the Spanish top-flight this season with 23 victories and seven draws in 30 outings.
Meanwhile, Roma had won four games in a row in all competitions before the 1-1 draw at Bologna on Saturday. However, they have six victories in their past eight Serie A fixtures.
The Giallorossi can take heart from their decent away record, with only one defeat in their past eight games on the road in all competitions.
Influential winger Cengiz Under misses out through injury for the visitors, while Sergio Busquets will face a late fitness test for Barca.
Barcelona: Ter Stegen; Semedo, Piqué, Umtiti, Jordi Alba; Busquets, Sergi Roberto, Rakitic, Iniesta; Messi, Luis Suárez.
AS Roma: Alisson, Peres, Fazio, Manolas, Kolarov, Strootman, De Rossi, Pellegrini, Florenzi, Dzeko, Perotti.