This will be the first time Ntshumayelo plays alongside Jali and Manyisa from the start in an official match since Bibo joined Pirates from SuperSport United in January.
Ntshumayelo started five League matches in a row for Bucs before he was relegated to the bench for the Soweto Derby when Jali returned from injury.
Kick-off for the clash at Nelson Mandela Bay Stadium is 20h00.