The action kicks off on Saturday afternoon when Chippa host Polokwane City at the Nelson Mandela Bay Stadium at 15h00.
The Chilli Boys will be without striker Lerato Manzini, who was sent off in their 4-1 demolition at the hands of Cape Town City on Monday evening, while Rise and Shine are forced to replace defender Thapelo Tshilo.
Ea Lla Koto, who come up against Orlando Pirates at the James Matlatsi Stadium on Saturday evening, will miss the services of Tamsanqa Teyise in central defence.
Meanwhile, First Division side Mbombela United will be minus left-back Mxolisi Mayongo when they take on Bloemfontein Celtic, who will have to do battle without Alfred Ndengane and Sibusiso Mxoyana at the Dr Molemela Stadium on Sunday afternoon.