The trio were all sent off in MTN8 action this week, with Masalesa running foul of the fourth official from the Bucs dugout in the 1-0 defeat to Ajax Cape Town.
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Matsatsantsa will start their League campaign against title rivals Pirates - who host CS Sfaxien in the CAF Confederation Cup on Saturday - at Peter Mokaba Stadium on August 11.
Wits begin their bid for Kaizer Chiefs' crown against Ajax on Saturday at Bidvest Stadium.
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If you planned to have any of these players in your KICK OFF Fantasy League teams for this week, you still have until an hour before kick-off on Saturday, 8 August to transfer them.