Due to a reported issue with flight tickets and visas, the Kenyan giants are only believed to have touched down at South Africa's OR Tambo International Airport at midnight on Tuesday.
Matsatsantsa, who reached the final of last year's Confed Cup, lost the first leg 1-0 in Nairobi.
The return duel is scheduled for 19h00 at Lucas Moripe Stadium.
SuperSport enter the match under the backdrop of fighting for survival in the Absa Premiership, as Kaitano Tembo's side are a point above the drop zone with three league games remaining.