Manyisa is expected to undergo surgery after fracturing his ankle in Pirates' goalless League draw with Polokwane City at Peter Mokaba Stadium last night.
"He [Manyisa] was released [from hospital] last night and travelled back with the rest of the team to Johannesburg. He is set to see a specialist today," says Bucs administrative manager Floyd Mbele.
The Buccaneers are also minus suspended defenders Happy Jele and Siyabonga Sangweni for the first leg tie at Orlando Stadium.
However, Mbele confirmed Sarr is available again. The Senegal international missed the team's previous two games after being granted compassionate leave following the death of his mother.