Forward Alexis Sanchez has been ruled out and academy prodigy Tanith Chong takes his place on the substitutes bench tonight.
The 29-year-old Chilean appeared as a late substitute in last Saturday's 2-2 draw at Chelsea but is "not fit", according to boss Jose Mourinho.
Juventus are set to start with Cristiano Ronaldo, who spent six years at Old Trafford between 2003-2009.
Manchester United: De Gea; Young, Smalling, Lindelof, Shaw, Matic, Pogba, Rashford, Martial, Mata, Lukaku.
Substitutes: Bailly, Pereira, Fred, Herrera, Romero, Darmian, Chong
Juventus: Szczesny; Cancelo, Bonucci, Chiellini, Lobo Silva, Bentancur, Pjanic, Matuidi, Cuadrado, Ronaldo, Dybala
Substitutes: De Sciglio, Benatia, Douglas Costa, Barzagli, Kean, Perin, Bernardeschi