The Premier League side remain one of three unbeaten teams in the European Cup this season and, with a credible record of late at the iconic Wembley Stadium, Spurs will feel confident of securing a result against Massimiliano Allegri's side.
After overturning a two-goal advantage at the Juventus Stadium through Harry Kane and Christian Eriksen to draw 2-2, the north London outfit have won three games and drawn one across all competitions.
Meanwhile, Juventus have enjoyed three consecutive 1-0 league victories in their quest for their seventh successive Scudetto.
Mauricio Pochettino's youthful outfit hold the slight advantage due to the away-goals rule, so the presence of Kane and Bianconeri club-record signing Gonazlo Higuian will be the key figures, as two of the most prolific goalscorers in Europe.
Toby Alderweireld remains the only notable absentee for the Lillywhites while Juventus are without wingers Juan Cuadrado, Federico Bernardeschi and striker Mario Mandzukic.
Tottenham: Lloris (C), Trippier, Sanchez, Vertonghen, Davies, Dier, Dembele, Eriksen, Dele, Son, Kane.
Juventus: Buffon; Barzagli, Benatia, Chiellini, Alex Sandro; Khedira, Pjanic, Matuidi; Dybala, Higuain, Douglas Costa.