The Spain international, who was an unused substitute during Real's derby win over the weekend, was seen remonstrating with teammates Marcelo and Luka Modric following the latest snub by his club boss.
Isco, who joined the Spanish giants from Malaga in 2013 and has been used sporadically by Zidane across the Frenchman's two spells at the Santiago Bernabeu, seemingly allowed his frustrations to get the better of him as he watched on in the stands.
"If he has to take me off, he takes me off in the 50th or 60th minute of the game, sometimes even at half-time," the 28-year-old was heard saying, according to AS h/t Movistar+. "If he has to put me on, he'll do it in the 80th minute."
Isco has made just two starts and two substitute appearances out of a possible seven this season, totalling 148 minutes on the pitch, and played no part in either of the LaLiga giants' latest two matches.