Ghana currently occupy second spot in the Group G standings behind South Africa and know that they will likely need to record back-to-back victories over Ethiopia and Bafana Bafana in order to reach the next round of qualification.
AS Roma starlet Felix Ohene Afena-Gyan has received a maiden call-up alongside Sheriff Tiraspol midfielder Edmund Addo, while Andy Yiadom, Mubarak Wakaso, Manaf Abdul Nurudeen and Richmond Boakye-Yiadom return to the squad.
Meanwhile, a number of familiar faces are also likely to feature, including brothers Andre and Jordan Ayew, Arsenal star Thomas Partey and Leicester City's Daniel Amartey.
Goalkeepers: Joseph Wollacott (Swindon Town), Lawrence Ati-Zigi (St.Gallen), Manaf Nurudeen (Eupen), Richard Attah (Hearts of Oak)
Defenders: Andy Yiadom (Reading FC), Philemon Baffour (Dreams FC), Baba Abdul Rahman (Reading FC), Gideon Mensah (Girondins Bordeaux), Daniel Amartey (Leicester City), Alexander Djiku (Strasbourg), Joseph Aidoo (Celta Vigo), Jonathan Mensah (Columbus Crew), Ismail Abdul Ganiyu (Asante Kotoko)
Midfielders: Baba Iddrisu (Real Mallorca), Thomas Partey (Arsenal FC), Mubarak Wakaso (Shenzhen F.C), Edmund Addo (Sheriff Tiraspol), Mohammed Kudus (Ajax Amsterdam), Daniel Kofi Kyere (FC St. Pauli)
Forwards: Andre Ayew (Al Sadd), Jordan Ayew (Crystal Palace), Abdul Fatawu Issahaku (Dreams FC), Kamaldeen Sulemana (Rennes), Samuel Owusu (Al Fayha), Richmond Boakye-Yiadom (Beitar Jerusalem), Benjamin Tetteh (Yeni Malatyaspor), Felix Afena Gyan (AS Roma), Caleb Ekuban (Genoa)
Ghana will play Ethiopia in Johannesburg on 11 November before returning home for the potential group-decider against Bafana Bafana three days later.