The west London giants had appealed to their supporters to come forward with any information of abuse following reports of violence after the Blues' 3-0 European Cup defeat to Barca at Camp Nou.
The Premier League side have since revealed an "unprecedented response" from their fans has led to them laying an official complaint with European football's governing body over Barca's security measures and crowd control.
A statement from Chelsea read: “We issued a plea for information to those fans who may have been involved in these incidents.
“This resulted in an unprecedented response, with a large number of individuals coming forward to complain in relation to poor organisation, lack of crowd control, brutality on the part of stewards and police and exposure to generally unsafe conditions.
“Chelsea takes the safety of our fans, both at home and away, extremely seriously, and this not what we expect when attending events organised by UEFA or its member clubs.
“We thank those supporters who shared their experiences to help form our report and will now await a response from UEFA.”