Sundowns skipper Hlompho Kekana is also doubtful for the Group C tie at Stade des Martyrs in Kinshasa, DR Congo (16h30).
The Brazilians, who drew their first pool match 0-0 with St George in Tshwane, released a statement on their website, which reads: 'Mamelodi Sundowns take on AS Vita from the Democratic Republic of Congo (DRC) in a crucial Champions League match, but might be without seven key players.
'Khama Billiat will sit this one out, as he’s still nursing an ankle injury.
'Captain fantastic, Hlompho Kekana is due for a late fitness test. The captain has a thigh strain.
'Tiyani Mabunda has not fully recovered from an ankle injury, which means he definitely misses the tie.
'Ricardo Nascimento has been out now for some time with a groin injury.
'Wayne Arendse is recovering from an ankle injury and has resumed gym work, after undergoing surgery.
'Asavela Mbekile is out with a foot injury.
'Bangaly Soumahoro is out with an ankle injury.'