The 58-year-old first took over the reins in 1999 although his time in charge of the Premier League was not without its controversies, including the tapping-up charge against Chelsea over Arsenal's Ashley Cole, as well as investigations into widespread corruption.
Scudamore said: It's an absolute privilege to have been allowed to enjoy this role for so long, it is too much fun to be called a job.
"Football is an intoxicating environment and therefore difficult to give up being so involved, but my passion for what football means to so many and the positive impact it can make will never be diminished.
"The past 20 years would not have been possible without the continuing support and encouragement of the clubs and the unstinting efforts of my colleagues at the Premier League, whose professionalism and dedication are only surpassed by their decency and sense of fun.
"We could not have achieved so much without the enormous commitment of broadcast and commercial partners across the world.
"That number will run into hundreds, they have all contributed to our story and I would like to thank them enormously."