Wilson, 26, earns his first call-up after seven Premier League goals this season, while Rooney will earn his 120th cap in a special one-off appearance for England against the US, following his retirement from international football.
The Cherries striker and Brighton defender Lewis Dunk are the two uncapped players included, while Derby's Mason Mount drop's out from the previous squad.
Everton centre-back Michael Keane is recalled to the squad for the first time in a year, with Leicester City's Harry Maguire missing out due to a knee injury.
Squad in full, as per the FA:
Goalkeepers: Marcus Bettinelli (Fulham), Jack Butland (Stoke City), Alex McCarthy (Southampton), Jordan Pickford (Everton)
Defenders: Trent Alexander-Arnold (Liverpool), Ben Chilwell (Leicester City), Lewis Dunk (Brighton), Joe Gomez (Liverpool), Michael Keane (Everton), Luke Shaw (Manchester United), Kieran Trippier (Tottenham), Kyle Walker, John Stones (Manchester City)
Midfielders: Dele Alli, Harry Winks, Eric Dier (Tottenham), Fabian Delph Manchester City), Jordan Henderson (Liverpool), Jesse Lingard (Manchester United), Ruben Loftus-Cheek, Ross Barkley (Chelsea),
Forwards: Harry Kane (Tottenham), Marcus Rashford (Manchester United), *Wayne Rooney (DC United), Jadon Sancho (Borussia Dortmund), Raheem Sterling (Manchester City), Danny Welbeck (Arsenal), Callum Wilson (AFC Bournemouth)
*USA fixture only