Volsteedt, who spent three years at Bucs, will link up with former Springbok coach Heyneke Meyer at French rugby club Stade Francais.
They previously worked together at the Blue Bulls in Tshwane.
“I would like to thank the Orlando Pirates family, the staff and especially the chairman [Irvin Khoza] for the support and encouragement over the past two-and-a-half seasons at the club,” Volsteedt told the club's website.
Pirates said in a statement: 'Orlando Pirates Football Club has amicably parted ways with the club’s head of strength and conditioning Andre Volsteedt.
'After three wonderful years at the club, Andre will be leaving the Buccaneers ship as he prepares for a new chapter in France.
'Andre departs for France where he will reunite with Heyneke Meyer who is the current head coach of Stade Francais. The two worked closely together at the Blue Bulls.
'The club would like to thank Andre and wish him all of the best in his future endeavours.'