This weekend Orlando Pirates will take on title rivals Kaizer Chiefs at FNB Stadium in what is South Africa's most hyped fixture.
And while the contest has largely failed to match expectations in recent years, Mosimane acknowledges the history of the two clubs and their large following cannot be denied.
Mosimane compared the two rivalries after the 1-1 Tshwane derby draw with SuperSport United at Lucas Moripe Stadium on Saturday.
"The game draws up to the occasion, it's the Tshwane derby," Mosimane said.
"For me it's the biggest derby. On the pitch it's the biggest derby and on the grandstand it's not the biggest derby.
"You must also understand how old is SuperSport United and you must also understand how old is Mamelodi Sundowns. We don't have the tradition of 1970, 69 or whatever years to build up this kind of derby.
"People of Tshwane must come and watch the Tshwane derby. It's the biggest derby. It was a good game."
Mosimane felt his men did well to create opportunities against what he termed the "reinforced defence" of Matsatsantsa.
"They had chances. They played the way they played and the way they played it guaranteed them at least a draw. It's a smash and grab. I don't want to use the other language. What is the right word? What is the modified word? Reinforced defence! That's the right word and play on the break. I think if they do this they get a point but we will see if they are consistent when they play away to get a point or was it because of the derby. They defended well.
"I think I must also give credit to my team. I think my team was very good. I am proud of my team. They played brilliant football. Maybe I am saying this because I like my team but I am still to see a team that plays football like this. We had by hook or crook to punch holes behind 10 defenders to be able to go through."
The table-topping Brazilians will be back in action against AmaZulu at King Zwelithini Stadium this Friday (18h00).