The defeat means AmaTuks extend their winless streak to six games, while Matsatsantsa claimed only their fourth victory of the campaign.
The game started off cagey, and dynamic winger Aubrey Ngoma created the first chance in the 22nd minute when he burst down the left flank but his shot was comfortably saved by Ronwen Williams.
Thokozani Sekotlong then had a brilliant chance to put his side in the lead five minutes before the break.
Williams had found himself in no man’s land after clearing a ball and was nearly caught out by a quick throw in from Ngoma but Sekotlong could not manage to lob the stranded Matsatsantsa keeper.
SuperSport came out the stronger side after the break, but it was Tuks again who created the first real chance of the second half.
A ball into the box found an unmarked Ngoma, but he failed to beat a rushing Williams.
Finally Gabonamong found the net, heading in a free-kick from Kermit Erasmus.
Warren Bishop nearly denied SuperSport the three points in the final minute of the match, but the defender’s header was saved by Williams.