Tottenham playmaker Erik Lamela has been rewarded with a first-call up since 2016 after his impressive form in the Premier League and is joined by club teammate Juan Marcos Foyth.
The former AS Roma stars' career has been blighted by injuries over the past couple of seasons and his return to the national team offers interim coach Lionel Scaloni a new dimension with his experimental squad.
Sevilla playmaker Franco Vazquez, who earned two caps for Italy, earns another call-up to the squad after his solid start to the new LaLiga season.
With Higuain, Messi and Aguero all left out, there's a chance for emerging stars Mauro Icardi, Paulo Dybala, Lautaro Martinez to all stake their claim for a starring role with the South American giants - who are looking to rebuild after their 2018 FIFA World Cup shortcomings.
Giovanni Simeone, son of Atletico Madrid manager Diego, is also included in the new-look La Albiceleste.
Goalkeepers: Agustin Marchesin (Club America), Sergio Romero (Manchester United), Geronimo Rulli (Real Sociedad)
Defenders: Ramiro Funes Mori (Villarreal), German Pezzella (Fiorentina), Renzo Saravia (Racing), Nicolas Tagliafico (Ajax), Walter Kannemann (Gremio), Nicolas Otamendi (Manchester City), Gabriel Mercado (Sevilla), Emanuel Mammana (Zenit), Juan Marcos Foyth (Tottenham)
Midfielders: Marcos Acuna (Sporting Lisbon), Maximiliano Meza (Independiente), Franco Vazquez (Sevilla), Giovani Lo Celso (Real Betis), Leandro Paredes (Zenit), Santiago Ascacibar (Stuttgart), Rodrigo Battaglia (Sporting Lisbon), Matias Zaracho (Racing), Roberto Pereyra (Watford), Rodrigo De Paul (Udinese), Franco Cervi (Benfica), Erik Lamela (Tottenham), Eduardo Salvio (Benfica)
Forwards: Angel Correa (Atletico Madrid), Lautaro Martinez (Inter Milan), Mauro Icardi (Inter Milan), Giovanni Simeone (Fiorentina), Paulo Dybala (Juventus)