Ronaldo, 33, has been accused of sexually assaulting Kathryn Mayorga at a Las Vegas hotel in 2009, an accusation that came to the fore in October last year through German publication Der Spiegel.
According to the weekly magazine, Ronaldo and the alleged victim reached an out of court settlement nine years ago with a $375,000 payment for agreeing never to go public with the allegations.
While the five-time Ballon d'Or winner has firmly denied the allegations, branding it 'fake news', Mayorga's lawyers are now seeking to declare the reported non-disclosure agreement void.
Now according to The Wall Street Journal, authorities sent a warrant to the court system in Italy, where Ronaldo is currently based in Turin - to identify if his DNA matches that of what was found on the dress of Mayorga.
"Mr Ronaldo has always maintained, as he does today, that what occurred in Las Vegas in 2009 was consensual in nature, so it is not surprising that DNA would be present, nor that the police would make this very standard request as part of their investigation," Ronaldo's lawyer, Peter Christiansen told the BBC.
Ronaldo moved to Juventus from Real Madrid for £99.2m in the off-season and is considered as one of the most recognised athletes in the world.
Breaking: Lawyer of Cristiano Ronaldo tells us it is “not surprising” after reports that Las Vegas police have issued a warrant to obtain the player’s DNA, after claims - which he denies - that he raped a woman in the US city 9 years ago, calling it a “very standard request”. pic.twitter.com/vjksc06T6K— Dan Roan (@danroan) January 10, 2019