Lamptey was in charge when Bafana Bafana beat Senegal 2-1 in their 2018 FIFA World Cup qualifier played at the Peter Mokaba Stadium in November 2016.
However, the match was subsequently ordered to be replayed after it was alleged that the match official had "manipulated" the result when a questionable penalty had been handed in favour of the hosts.
In a statement released by the world football governing body on Monday, it was confirmed that Lamptey's appeal to the Court of Arbitration for Sport had been thrown out.
"FIFA has taken note of the motivated arbitral award of the Court of Arbitration for Sport (CAS) confirming the lifetime ban imposed by FIFA’s Disciplinary and Appeal Committees on Ghanaian match official Joseph Odartei Lamptey," read the statement.
"FIFA’s judicial bodies had banned Mr Lamptey for life for breaching art. 69 par 1 (unlawfully influencing match results) of the FIFA Disciplinary Code during the 2018 FIFA World Cup Russia™ qualifying match between South Africa and Senegal on 12 November 2016.
"In its ruling, CAS concluded that Mr Lamptey had intentionally taken two wrong decisions with the sole purpose of enabling a specific number of goals to be scored that would make pertinent bets successful.
"CAS concluded that there was an obvious link between these intentionally wrong decisions and a deviation from an expected betting pattern, and consequently found Mr Lamptey guilty of having unlawfully influenced the result of the match.
"This CAS decision underlines FIFA’s commitment to protecting the integrity of football and its zero-tolerance policy on match manipulation, while also highlighting the effectiveness of its current agreement with Sportradar that uses their Fraud Detection System, which played an important role in this case."