The 37-year-old ex-Pirates midfielder has spent the past two years at Mayfair learning his craft under Muhsin Ertugral, Kjell Jonevret and current head coach Milutin 'Micho' Sredojevic, and also had a stint in charge of the reserve side.
Mhlongo will now look to make his mark at Mpumalanga-based Sporting, who gained promotion to the second tier via the ABC Motsepe League play-offs.
Pirates released a statement, which reads: 'Benson Mhlongo has received the chairman Dr Irvin Khoza’s blessings to leave the club and pursue his dream of becoming a head coach.
'After spending two seasons as assistant coach at the club, the 37-year old is now ready to make the jump to assume a head coaching role at newly promoted National First Division outfit TS Sporting.
'Dr Khoza granted Mhlongo’s wish to leave the club following a meeting with chairman of TS Sporting Slungu Thobela earlier this week.
'Mhlongo leaves the club having amassed a wealth of knowledge where he was exposed to three different head coaches at the Club, including a stint as head coach of the Orlando Pirates MultiChoice Diski Challenge team.
'On behalf of the Orlando Pirates family we would like to wish Benson the best of luck in his future endeavours.'
Micho still has main assistant coach Rhulani Mokwena at his disposal in a technical team that includes goalkeeper coach Andrew Sparkes, head of performance Franck Plaine and performance analysts Darian Wilken, Kyle Solomon and Michael Loftman.