The 23-year-old hurt his shoulder in a 1-0 victory over Polokwane City in the Premiership on Wednesday night.
Lehlogonolo Masalesa, who has impressed in Jali's absence before, is now likely to partner Oupa Manyisa in the middle of the park at Orlando Stadium.
Thandani Ntshumayelo has recovered from a lengthy ankle problem, but is still short of match fitness.
Bucs, with seven points, share second spot in Group A with AC Leopards - three points behind leaders Ahly, and three points ahead of bottom-placed Zamalek heading into their final pool match.
The top two sides will advance to the semi-finals of the competition.
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