French-born midfielders Jamel Saihi from Montpellier and Fabien Camus are drafted back after Tunisia were held to a goalless draw at home in Tunis last month in the first-leg.
Saihi returns from after a long term injury while Camus, who plays at Racing Genk in Belgium, gets a recall four years after his only previous cap.
There is a first call up for Stephane Nater, another French born player with Tunisian roots.
Krol has added more attacking options with the inclusion of Ahmed Akaichi from Esperance while defenders Mohamed Ben Mansour and Bassem Boulaabi and youthful striker Edem Rjaibi are also called up for the first time.
Goalkeepers: Moez Ben Cherifia (Esperance), Rami Jeridi (CS Sfaxien), Aymen Mathlouthi (Etoile Sahel)
Defenders: Mohamed Ben Mansour (Esperance), Siam Ben Youssef (FC Astra), Bassem Boulaabi (CS Sfaxien), Alaya Brigui (Etoile Sahel), Khalil Chammam, Sameh Derbali, Chamseddine Dhaouadi (all Esperance), Yassine Mikari (FC Luzern), Alaeddine Yahia (Racing Lens)
Midfielders: Anis Ben Htira (Hertha Berlin), Wissem Ben Yahia (Mersin Idmanyurdu), Fabien Camus (Rcaing Genk), Yassine Chikhaoui (FC Zurich), Stephane Nater (St Gallen), Houcine Ragued (Esperance), Jamel Saihi (Montpellier), Ferjani Sassi (CS Sfaxien)
Forwards: Ahmed Akaichi (Esperance), Sami Allagui (Hertha Berlin), Fakhreddine Ben Youssef (CS Sfaxien), Amine Chermiti (FC Zurich), Saber Khalifa (Olympique Marseille), Edem Rjaibi (CA Bizerte)