The two-legged tie will see the South Africans face off against The Zebras at the Lobatse Sports Complex Stadium on 9 April, while the return leg is scheduled for Makhulong Stadium in Tembisa on 12 April.
Head coach Vera Pauw has selected 28 players for the camp, with Busisiwe Ndimeni ruled out because of an injury she sustained in Cameroon, while Jabu Mazibuko returns to the squad after missing out on the West African travel due to an injury.
The South Africans played two international friendly matches against the host nation – winning 2-0 in the first one and drawing 2-2 in the second.
Banyana will conduct their training sessions at the Nike Football Training Centre in Pimville, Soweto before departing for Botswana on Tuesday.
“We have just recently returned from Cameroon where we did very well, and we are now looking forward to these upcoming clashes with Botswana,” Pauw says.
“Our travel to West Africa has helped us with preparations for Botswana because we really want to qualify for the AWCN and make the nation proud. All these matches that we are playing are part of the programme to prepare for the bigger stage, the Rio Olympic Games in August.”
Cameroon will host the 12th edition of the AWCN tournament from 19 November to 3 December, 2016.