Uzoho, 20, made his senior international debut for the Super Eagles in October last year.
He's since emerged as the African giants first-choice stopper, amassing 13 caps since his bow during the 4-2 win over Argentina.
And his absence will be seen as a major blow after Spanish outfit Elche confirmed he'll be out for a month due to an untimely rib injury.
The eccentric keeper has been replaced by Enyimba's Theophilus Afelokhai for the upcoming encounter against Stuart Baxter's side on 17 November.
Injury has also ruled out experienced Ogenyi Onazi, along with Wilfried Ndidi and John Obi Mikel's absence due to suspension and leave of absence respectively - ahead of the fierce encounter with Bafana Bafana at the FNB Stadium.
Victory for either side will seal qualification to next year's continental showpiece in Cameroon.
Nigeria 23-man squad:
Goalkeepers: Theophilus Afelokhai (Enyimba); Ikechukwu Ezenwa (Enyimba); Daniel Akpeyi (Chippa United, South Africa)
Defenders: Olaoluwa Aina (Torino, Italy); Adeleye Aniyikaye (FC IfeanyiUbah); Semi Ajayi (Rotherham United, England); Bryan Idowu (Lokomotiv Moscow, Russia); William Ekong (Udinese, Italy); Leon Balogun (Brighton & Hove Albion, England); Kenneth Omeruo (Leganes, Spain); Jamilu Collins (SC Padeborn, Germany)
Midfielders: Oghenekaro Etebo (Stoke City, England); John Ogu (Hapoel Be'er Sheva, Israel); Mikel Agu (Vitoria Setubal, Portugal)
Forwards: Ahmed Musa (Al Nasr, Saudi Arabia); Kelechi Iheanacho (Leicester City, England); Moses Simon (Levante, Spain); Victor Osimhen (Royal Charleroi SC, Belgium); Odion Ighalo (Changchun Yatai, China); Alex Iwobi (Arsenal, England); Samuel Kalu (Bordeaux, France); Isaac Success (Watford, England); Samuel Chukwueze (Villarreal, Spain)