The trials, which have been agreed to by the FA, EFL and Premier League, will begin tonight at Falmer Stadium and will see a team of two officials watching the game on screens to help the match referee make correct decisions.
The referee at the game can then request help from VAR, or even view an incident himself on a pitch-side screen, while the video referees can also inform the official of any missed action.
"The message to players and supporters is clear: Minumum interference, maximum benefit," head of referees Mike Riley told reporters.
"Our research has indicated four per cent of on-field decisions are incorrect, we hope VAR can get that down to 2%.
"But it's worth remembering this is a trial, it's not perfect and everyone is learning here. The referees are new to this."