Kick-off is at 20h00 at Lucas Moripe Stadium in Atteridgeville, Tshwane.
Both sides have four points from two games - two points more than St George, while bottom-placed AS Vita Club are yet to open their account.
“Our campaign so far has been fairly okay, as we have been able to get four points from our two games,” Vilakazi told his club's website, referring to a 3-1 win over Vita in DR Congo and goalless draw with Ethiopian visitors St George.
“This game will be crucial for us. It is important that we use our home ground advantage, and we have prepared well for the outing. Everyone is ready, and anyone selected to play can do the job for the team, fans and country.
“Esperance is a big team, and have a great history in continental contests, and have been dominant, so it is not going to be easy. But we know we are capable of getting all three points. All the players are craving to get to the top and remain there until the end of the round.
“It’s exciting to get the opportunity to play in the Champions League. It is a deep feeling, and we want to do it again.”