The African champions will first meet the Antelopes inside the National Stadium on 12 October.
The teams will then contest the return duel at the U. J. Esuene Stadium in Calabar on 16 November.
Headlining the squad are stand-in skipper Vincent Enyeama, England-based midfielders Mikel Obi and Victor Moses, and forwards Brown Ideye and Emmanuel Emenike.
Coach Stephen Keshi has called up three goalkeepers, seven defenders, seven midfielders and six strikers.
The Nigeria Football Federation is making arrangements to have the squad train for four days in Abuja heading into the opening encounter.
Super Eagles squad
Goalkeepers: Vincent Enyeama (Lille FC, France); Austin Ejide (Hapoel Be’er Sheva, Israel); Chigozie Agbim (Enugu Rangers, Nigeria).
Defenders: Elderson Echiejile (Sporting Braga, Portugal); Francis Benjamin (Heartland FC, Nigeria); Efe Ambrose (Celtic FC, Scotland); Solomon Kwambe (Sunshine Stars, Nigeria); Godfrey Oboabona (Rizespor FC, Turkey); Azubuike Egwuekwe (Warri Wolves, Nigeria); James Okwuosa (Chippa United, South Africa).
Midfielders: John Mikel Obi (Chelsea FC, England); John Ogu (Academica de Coimbra, Portugal); Ogenyi Onazi (SS Lazio, Italy); Nosa Igiebor (Real Betis, Spain); Victor Moses (Chelsea FC, England); Sunday Mba (Enugu Rangers, Nigeria); Nnamdi Oduamadi (AC Milan, Italy).
Forwards: Ahmed Musa (CSKA Moscow, Russia); Brown Ideye (Dynamo Kyiv, Ukraine); Shola Ameobi (Newcastle United, England); Emmanuel Emenike (Fenerbahce FC, Turkey); Obinna Nsofor (Lokomotiv Moscow, Russia); Uche Nwofor (SC Heerenveen, Netherlands).