Former Cup holders Dolphins lost 4-2 on penalties to amateur side Akwa Starlets in Umuahia, after both sides failed to break the deadlock after 90 minutes. Another Port-Harcourt club Sharks FC were nearly handed a shock exit by Warri-based amateur side J. Atete FC as they won 6-5 on penalties after both teams failed to score during regulation time
Oghogho Oduokpe's brace helped Rangers record a 2-0 win over Samba Kuma FC, while Gbolahan Salami's goal helped Shooting Stars record a slim 1-0 win over JUTH FC.
Akwa United and Warri Wolves also recorded 1-0 wins over Makwanda FC and Spotlight FC respectively. But Glo Premier League side Kaduna United bowed out of the competition after losing 2-1 to NNL side Abia Warriors FC.
Kano Pillars beat El Kanemi 2-1 in a battle of Premier League teams in Lafia. ABS FC also beat Bayelsa United 5-4 on penalties in Benin City, while Sunshine Stars beat Nembe City 2-1 in Nnewi.
In the second played at Nnewi last year's finalists Lobi Stars beat MFM FC 2-0, while defending champions Heartland FC beat Ingas FC 1-0 in Enugu.
Nasarawa United recorded the biggest win after beating COD United 4-0 in Ilorin, while Enyimba defeated Remo Stars 2-1 at the Confluence Stadium in Lokoja.
Niger Tornadoes beat Plateau United 6-5 on penalties, while Gombe United beat Taraba FC 2-0 in the second game played in Lafia.
ROUND OF 32 RESULTS:
Juth FC 0-1 Shooting Stars- Niger
Heartland FC 1-0 Ingas FC – Enugu
J. Atete FC 0-0 Sharks FC (PK 6-7) – Abakaliki
Akwa United FC 1-0 Makwada FC – Katsina Ala
Enugu Rangers 2-0 Samba Kurna FC – Abuja
Plateau United 0-0 Niger Tornadoes (PK 5-6) – Kaduna
Warri Wolves 1-0 Spotlight FC – Ilorin
El-Kanemi Warriors 1-2 Kano Pillars – Lafia
FC Taraba 0-2 Gombe United – Lafia
Nasarawa United 4-0 COD FC – Ilorin
Nembe City 1-2 Sunshine Stars – Rojenny
Remo FC 1-2 Enyimba FC – Lokoja
Dolphins FC 0-0 Akwa Starlet (PK 2-4) – Umuahia
Abia Warriors 2-1 Kaduna United – Lokoja
ABS FC 0-0 Bayelsa United (PK-5-4) – Benin City
MFM FC 0-2 Lobi Stars – Nnewi