Mrs Dilichukwu Onyedinma, who heads the Women's League body, announced after the Annual General Assembly, that the practice is now officially banned.
According to Onyema, players found to be in contravention would be dismissed and also barred from representing Nigeria
“Any player that we find is associated with it will be disqualified,” she added.
“We will call the club chairmen to control their players, and such players will not be able to play for the national team.”
Onyema said the League body will work with clubs to stop the practice.
“It (lesbianism) is happening but we have to talk to the clubs, and look inside the clubs and these things have to do with clubs.
“There are particular clubs that don't even want to hear about it and once they heard it the players involved will be sacked,” Onyema, a member of the NFF executive committee, said.
Onyema's comments come two years after Super Falcons coach Eucharia Uche declared that the team were performing better because she had rid the squad of lesbians.