Amakhosi take on the Urban Warriors at Peter Mokaba Stadium.
Stuart Baxter says he is having a tough time getting his players focused on the Ajax match, with the Soweto Derby against Orlando Pirates looming large this weekend.
"The game against Ajax Cape Town is a doubly tough game for us. It is important that both our eyes are fixed on the first game and that is the focus for now. We cannot afford to think about the game over the weekend," the coach told kaizerchiefs.com.
"It can be a bit of a distraction when you have a game ahead of the Derby, which makes the game at hand even more important and tougher. I have to continue addressing this with the players as we continue to prepare for Ajax game."
Meanwhile, Baxter says the return of 'Tower' and 'Yeye' is a boost for Chiefs, and Mathoho says he will be relieved to be back on the pitch, after watching his team lose against Platinum Stars recently.
"It hurt to watch the team lose against Platinum Stars," Mathoho says. "That is now water under the bridge. We are all determined to bounce back against Ajax Cape Town. We want to start the festive season on a high note and it means we have to beat them."