Kick-off is at 3pm.
The Urban Warriors suffered a second successive defeat in midweek when they went down 2-0 away to Orlando Pirates. Their only win of the season so far was the 4-1 home victory over Platinum Stars in late August
Ajax are 13th on the log with four points from five matches.
Meanwhile, University of Pretoria kept up their unbeaten record this season with a goalless draw at home to Platinum Stars at the weekend.
They have won both of their two away matches so far this season (against Golden Arrows and Moroka Swallows) and will be hoping to complete a hat-trick against Ajax.
AmaTuks are fifth on the log with nine points from five matches.
This will be the first competitive League meeting between Ajax Cape Town and University of Pretoria.
Ajax's line-up from their 2-0 loss to Pirates: Westerveld, Lebusa, Allie, Booth, Jenniker, Rusike (van Graan 67), Scott, Dolly (Manyama 46), van de Laak (Shabalala 78), Billiat, Grobler.
Tuks’ line-up from their 0-0 draw with Stars: Arubi, Mphahlele, Shoyisa, Sibande, Mkhwanazi (Monyai 47), Matsi, Maruping (Ngwenya 39), Mutizwa, Ngoma, Mosadi (Selolwane 83), Niang.