South Africa beat Mozambique 2-0 on Tuesday, courtesy of a brace from Bernard Parker, and Matlaba came on in the 86th minute to replace Siphiwe Tshabalala.
The fullback, who is currently considered one of the best in his position in the PSL, says he is not concerned about his limited time on the pitch, saying it was "the best seven or so minutes ever."
The call-up was Matlaba's second, the first having been against Tanzania in May.
"The experience of being in the national team is priceless, I just cannot explain it. It is my second season at Pirates and I am glad that my performances at club level have not gone unnoticed," the 25-year-old tells Bucs' official website.
"I am grateful to the coach and his technical team for having the confidence in me and for giving me an opportunity to play a part of the game.
"What is important now is that I remain consistent at my club and show that I'm not a one hit wonder. It is simple – if I play well for Pirates then I stand a very good chance of getting another call-up to the national team," he says.