There are no surprises in the starting XI, which features key playmaker Neymar, while Barcelona right-back Dani Alves has overcome a thigh strain to start.
The Chelsea trio of centre-back David Luiz, and midfielders Ramires and Oscar both start, along with the highly-rated Sao Paulo star Lucas Moura, who will play at his club's home ground.
Vasco da Gama defender Dede will partner David Luiz at centre-back, with Real Madrid left-back Marcelo next to them.
Spartak Moscow winger Romulo has also been included, while up front will be Neymar and Internacional striker Leandro.
Valencia goalkeeper Diego Alves will be the player that Bafana will have to beat between the sticks.
The line-up is fairly inexperienced at this level, with just 170 caps between them, an average of 15 per player.
The match in Sao Paulo kicks off at 20h45 South African time and will be live on Sabc 1.