The changes were ratified at a recent Board of Governors (BOG) meeting and relate to suspensions, disputes and medical attention at fixtures.
The League released a statement saying the threshold for yellow-card suspensions has been extended from three bookings to four, after which the player will receive a one-game ban.
A player will receive a further one-match suspension after another four bookings; while a further four yellow cards will then draw a three-game ban.
There had been general unhappiness that the previous rules of just three cards before a one-match suspension was too strict.
In another change, a time limit for lodging of complaints has been introduced. This means that complaints must now be lodged within 40 days of the closing of the previous transfer window.
The PSL has also beefed up medical attention at matches.
"Following the incidents involving Fabrice Muamba and Itumeleng Khune, the League has introduced a detailed set of medical rules that clubs will be expected to adhere to in the future," reads the statement.
"According to the new rules, the home club will have to provide a doctor and physiotherapist at every match to take care of the players and match officials, and an additional doctor with supporting paramedics to deal with any medical incidents in the crowd."
"The Rules also specify minimum levels of equipment and medical facilities that must be available at all times including, but by no means limited to, a defibrillator and oxygen."