Both players were said to have been signed to contracts recently, but a club insider has confirmed that this is not the case.
Booth is holding out for a longer deal than the 12 months that were offered to him by the club and will re-start negotiations when club CEO George Comitis returns from a holiday in Greece on July 18.
In the meantime he has been training with the club and participating in pre-season friendlies in case a deal can be struck.
Isaacs has been training the with team for the past few weeks and although it was reported in the media earlier this week that he had put pen to paper on a new deal, KickOff.com understands this is not the case.
It means at present the club’s centre-back options include two converted right-backs, Nazeer Allie and Thulani Hlatshwayo, Thato Mokeke and youngster Nathan Gertse who has no PSL experience.
The uncertainty over the future of the pair has reignited the club’s interest in Orlando Pirates defender Robyn Johannes, who wants to return to Cape Town for family reasons.
He would welcome the move, but Ajax have made it clear that they will not sign all three of the players, and so he will have to wait on the outcome of the negotiations with Booth and Isaacs before his future becomes clearer.