Despite losing 3-2 against Mpumalanga Black Aces, AmaTuks are six points clear of second placed Blackburn Rovers with one game left to play.
Two first half goals from Phumelele Bhengu and one in second from Liniker Nwikisa gave Aces the much-needed three points, but they are still not out of the relegation zone with 30 points.
Atlie FC had a great start to the game when Phumudzo Manenzhe scored in the first minute to give them the lead against Sivutsa Stars. However, they could not hang on to the lead and let in a late goal from Sakhile Mkhalipha in the 75th minute to level the scores.
Atlie stay bottom with 27 points, while Sivutsa move to 12th.
Bay United beat Witbank Spurs 1-0, and stay in the relegation zone. United are in 14th place with 31 points, while Spurs move down to 11th with 34.
Dynamos also got a much needed win when they beat FC AK 4-3 at home. Dynamos are now on 31 points while FC AK's chances of a Play-off place hit a stumbling block, as they move down to fifth.
Chippa United broke Blackburn Rovers' hearts when they beat the title contenders 2-0. Rovers will not get automatically promoted to the Premiership, but are still in with a chance of a Play-off place.
United occupy the last Play-off place with 48 points, while Rovers stay second with 49.
Thanda Royal Zulu kept the pressure up on Chippa, who are currently third. Thanda thashed Vasco da Gama 4-1 to move to 47 points. Vasco remain sixth with 42 points and cannot make it to the Play-offs.
FC Cape Town beat United FC 1-0 and remain one above United in eighth.
Carara Kicks jumped to 10th place when they beat African Warriors 2-1.
With the promotion spot having been decided, focus will now change to the Play-off spots, as well as the bottom of the table, where four teams still have a chance of being relegated with just one game left to be played next weekend.